P67

AP-1 is essential for p67 promoter activity

The cytosolic NADPH oxidase cofactor p67 has been shown to be one of the limiting factors in assembly and activation of this multiprotein enzyme complex and, therefore, must be highly regulated at the transcriptional level. In the present studies, we have further characterized the promoter for human p67. Genomic sequence upstream of the translational start site (TLS; 2 kb) was cloned, and RACE ...

Cloning and expression of P67 protein of Mycoplasma leachii

AIM The present study was undertaken to clone, express and study the immunogenicity of P67 protein of Mycoplasma leachii. MATERIALS AND METHODS P67 gene was amplified from genomic DNA of M. leachii. The polymerase chain reaction (PCR) product was inserted in pRham N-His SUMO Kan vector and was used to transform competent Escherichia cloni 10G cells. Recombinant protein expression was done by ...

P67 - Mothers restrict physical activity for children and teens asthmatics

Methods In this cross sectional study were included 115 asthmatic children and adolescents 9 to 19 years old and their mothers. For the children, asthma severity, PA level and exercise induced bronchospasm (EIB) were evaluated. The mothers were investigated on their beliefs about PA in non-asthmatic and asthmatic children, if they imposed restrictions to their children PA, perception of exercis...

HoxA10 Represses Transcription of the Gene Encoding p67 in Phagocytic Cells

Two-exon skipping due to a point mutation in p67-phox--deficient chronic granulomatous disease.

The cytosolic 67-kD protein in phagocytes (p67-phox) and B lymphocytes is one of essential components of the superoxide-generating system in these cells, and its defect causes an autosomal recessive type of chronic granulomatous disease (CGD). We performed mutation analysis of p67-phox mRNA from a CGD patient who lacks the protein and found an in-frame deletion from nucleotide 694 to 879, which...